不同幽门螺杆菌临床分离株的菌体蛋白

STUDY ON THE PROTEINS OF DIFFERENT CLINICAL Helicobacter pylori ISOLATES

目的：了解不同幽门螺杆菌的菌体蛋白构成及其免疫印迹条带与胃十二指肠疾病的关系。方法：将６株幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ，Ｈｐ）临床分离株的菌体蛋白进行十二烷基磺酸钠聚丙烯酰胺凝胶电泳（ＳＤＳＰＡＧＥ），以免疫印迹技术分析其免疫原性；以６株Ｈｐ菌体蛋白的等量混合物为抗原，检测了６２例胃十二指肠疾病患者血清抗ＨｐＩｇＧ的免疫印迹条带。结果：凝胶上６株Ｈｐ的菌体蛋白条带非常相似，均含６７．０ｕ、６５．０ｕ、５７．０ｕ３条主要蛋白条带，但各主要蛋白条带的含量在６株Ｈｐ中各不相同（薄层扫描法）；除共同含有６７．０ｕ、５７．０ｕ、５５．０ｕ、５２．０ｕ４条免疫反应条带外，６株Ｈｐ的其余免疫反应条带的检出率及颜色深浅有明显差异；２４．８ｕ免疫反应条带在胃癌组的检出比例（１１／１２）显著高于胃溃疡组（４／１３）、十二指肠溃疡组（７／１４）和慢性胃炎组（１／１２）。结论：不同Ｈｐ临床分离株的菌体蛋白结构相似，但其蛋白含量和免疫原性有差异；２４．８ｕ蛋白可能与胃癌有相关性。

Objective:To observe the proteins of different Helicobacter pylori (Hp) clinical isolates and their immunogenicity.Methods:The proteins were analyzed by SDSPAGE and immunoblot.Results:(1)The protein profiles of six different clinical Hp isolates confirmed by SDSPAGE were very similar, the same major protein bands showed different quantity through thinlayer scanning analysis in different Hp isolates. Immunoblot test showed that all six clinical Hp isolates contained 67.0 u, 57.0 u, 55.0 u, 52.0 u immunoreactive bands, however, the presence of other immunoreactive bands were significantly different and there was not any regulation. (2) The serum was analyzed by immunoblot method for recognizition of Hp protein band in 62 patients with various gastroduodenal diseases. The result showed that 51 were tested positive with the detection rate of 79.5 u protein band ranged from 6/12 to 11/12, and there were no significant differences among these groups. The frequency of 24.8 u protein band was 11/12 in gastric cancer group, significantly higher than that in peptic ulcer group and chronic gastritis group.Conclusion:The result indicated that there were some differences in Hp protein quantity and immunogenicity among these different clinical Hp isolates, and 24.8 u protein probably correlated with gastric cancer.