实验性胃溃疡大鼠胃粘膜中IL-8的免疫组织化学研究

Study of immunoreactive interleukin8 in the gastric mucosal cell of stress and indomethacininduced ulcer rats

目的：探讨在应激和注射消炎痛诱导的胃溃疡大鼠胃粘膜白细胞介素８（ＩＬ８）免疫反应阳性物质含量的变化．方法：将浸水应激、注射消炎痛大鼠各自分为０５，１，２和４ｈ组，取胃窦标本进行免疫组化染色，并以图像分析方法分析各个时间点胃粘膜细胞中ＩＬ８免疫阳性物质含量的变化．结果：与正常对照组相比，在浸水应激、注射消炎痛０５ｈ时大鼠胃粘膜细胞内ＩＬ８免疫反应阳性物质含量明显降低，随着时间延长ＩＬ８含量水平逐渐回升，４ｈ时明显高于０５ｈ时，但仍较正常对照组为低．结论：浸水应激或消炎痛性胃溃疡形成早期，胃窦部粘膜细胞内ＩＬ８一过性被释放到细胞外，同时可能由于胃粘膜细胞中ＩＬ８的合成增强，导致粘膜细胞内ＩＬ８含量水平回升．

Aim: To investigate the change of immunoreactive IL8 in the rat gastric mucosal cell during stress and indomethacininduced ulcer Methods: Immunocytochemical ABC method and image analysis were applied Results: Half hour after cold water stress or indomethacin administration, the quantity of immunoreactive IL8 dropped markedly in the gastric mucosal cell of ulcer rats comparing with normal rats, then rose again slowly with the time elapsing Conclusion: In the early stage of ulcer, IL8 was secreted from the mucosal cell transiently and at the same time, the synthesis of IL8 may be strengthened The role of IL8 in the development of ulcer and its mechanism need further study