RP-HPLC测定调血降脂丸中丹参酮Ⅱ_A的含量

Determination of TanshinoneⅡ_A in Tiaoxuejiangzhi Pill by RP-HPLC

目的：建立调血降脂丸中丹参酮ⅡA的含量测定方法。方法：采用反相高效液相色谱法测定含量。色谱柱为DiamonsiL C18;流动相为甲醇-水（87∶13）;流速为1.0 mL.min^-1;检测波长为270 nm。结果：丹参酮ⅡA在（0.11-0.65）μg范围内与峰面积成良好的线性关系（r=0.999 1）;平均加样回收率为100.1%（n=5）,RSD=0.3%。结论：该法灵敏、准确,重复性好,可作为调血降脂丸中丹参酮ⅡA的含量测定方法。

Objective：To develop a RP-HPLC method for the determination of tanshinone ⅡA in Tiaoxuejiangzhi Pill. Medthod：The determination was performed on a DiamonsiL C18 column（250 mm × 4.6 mm, 5μm）. The mobile phase was a mixture of methanol-water（87：13）, the flow rate was 1.0 mL.min^-1 and the detection wave length was at 270 nm. Result：The linear ranges of tanshinone ⅡA were at（0.11 - 0.65）μg （ r = 0.999 1） ;The average recovery was 100.1%, RSD = 0.3 % （ n = 5 ）. ConcLusion： The method is sensitive, accurate arid reproducible. It can be used to determine the content of tanshinone ⅡA in Tiaoxuejiangzhi Pill.