清脑宣窍滴丸对急性脑缺血损伤大鼠的保护作用及机理初探

Protective Effect of Qingnao Xuanqiao Dropping Pill on Experimental Ischemic Injury

目的:研究清脑宣窍滴丸对急性脑缺血损伤的保护作用及机理。方法:采用线栓法所致大鼠大脑中动脉缺血再灌注(MCAO)模型,测定大鼠脑梗塞范围;测定脑匀浆中乳酸(LD)、丙二醛(MDA)、谷胱甘肽(GSH)含量及乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活力;应用免疫组化染色的方法,观察清脑宣窍滴丸对黏附分子-1(ICAM-1)表达的影响。结果:清脑宣窍滴丸180 mg.kg-1、90 mg.kg-1可明显减小MCAO大鼠脑梗塞范围;180 mg.kg-1可明显降低MCAO大鼠患侧脑匀浆中LD含量,180 mg.kg-19、0 mg.kg-1、45 mg.kg-1可明显降低手术侧大脑组织中升高的LDH活力、提高SOD活力、降低MDA含量,180 mg.kg-1、90 mg.kg-1可明显提高手术侧大脑组织中GSH含量;180 mg.kg-1可显著降低ICAM-1阳性表达的积分光密度(IOD)。结论:清脑宣窍滴丸对脑缺血有保护作用,可能机理是通过减轻酸中毒、抑制自由基损伤、恢复自由基代谢平衡,抑制黏附分子表达来减轻缺血再灌注后炎症反应。

Objective：To study the role and mechanism of Qingnao Xuanqiao Dropping Pill（QNXQDP） on acute cerebral ischemia. Methods： Adopting the middle cerebral artery occlusion （MCAO）model induced by inserting a monofilament suture, the infarct size, the content of LD, MDA and the activity of GSH and LDH, SOD in brain homogenate were observed. We determined the expression of ICAM-1 in the brain influenced by QNXQDP by immunohistochemical staining method. Results： QNXQDP 180 mg.kg^-1,90 mg.kg^-1 can significantly reduce the scope of the rat MCAO cerebral infarction; 180 mg.kg^-1 could significantly reduce the content of LD in the rat brain homogenate of the troubled side, 180 mg.kg^-1, 90 mg.kg^-1, 45 mg.kg^-1 can significantly reduce the increased LDH activity, enhance the activity of SOD and reduce the content of MDA in the side of the brain surgery, 180 mg.kg^-1, 90 mg.kg^-1 can significantly improve the content of GSH in the side of the brain operation; 180 mg.kg^-1 can significantly reduce the integral optical density of ICAM- 1 in positive expression. Conclusion： QNXQDP has protective effect on cerebral ischemia. The possible mechanism may be by reducing acid poisoning, inhibiting free radical damage and restoring the balance between free radical metabolism; inhibiting the expression of adhesion molecules, reducing the inflammatory response induced by ischemic repeffusion.