佐匹克隆的薄层荧光测定及在中毒家兔体内的分布研究

TLCF Determination of Zopiclone and on Distribution of the Drug in Poisoned Rabbits

应用薄层荧光扫描法测定了动物血浆中佐匹克隆的浓度，并对佐匹克隆在中毒家兔体内的分布进行了研究。样品在ｐＨ６８６条件下用二氯甲烷—异戊醇（９８∶２）提取，氯仿—甲醇（９∶１）展开，用ＣＳ—９３０薄层扫描仪在激发波长３０５ｎｍ进行荧光单波长线性扫描测定。本法对血浆中佐匹克隆的最低检出限为２５ｎｇ／ｍＬ，平均提取回收率８０％，ＲＳＤ为２０４％。研究发现，急性中毒家兔１２ｈ后其胃内容物、肝、脑和胆汁中佐匹克隆含量较高。

A method is reported for the detection and analysis of zopiclone concentration in plasma by thin-layer chromatography-fluorometry and used to study the distribution of zopiclone in poisoned rabbits.The samples were adjusted to pH 686,extracted with dichloromethane-isoamyl alcohol(98∶2)and developed with chloroform-methanol(9∶1).The plate was scanned at an excitation wavelength of 305nm and number two emission filter plate in the fluoresence linear scan mode of CS-930.The average recovery was 80% and RSD was 204%.Its limit of detection in plasma was 25ng/mL.The concentrations of zopiclone in gastric contents,liver,brain and bile were higher than these in other tissues when the rabbits were poisoned after 12 hours.