摘要
目的:研究外周血干细胞移植时,流式细胞仪在干细胞含量检测方面的应用。方法:用CD34单抗标记动员后CS-3000plus分离的外周血单个核细胞。用Facscalibur中的Cellquest软件收集分析。结果:正常人外周血中CD34+细胞从动员前的0.02%~0.22%上升到1.21%~2.47%。受者输入的CD34+量为3.71~11.8×106个细胞/千克受者体重。结论:流式细胞仪为临床提供了一种快速简捷的干细胞测定方法。
Objective: Research the use of flowcytometry(FCM) in allo-peripheral blood stem cell transplantation(PBSCT).Methods:donors' peripheral mononuclear cells which were marked CD34 antibody after moblilizationg were acquired andanalysed with cellquest version 1. 2. 2 software in Facscalibur. Results: concentration of Co34+ cells in healthy donors peripheral flood increased from normal level 0.02%~0.22% to 1 .21% ~ 2.47% and that of infusion CD34+ cells are3.71-11 .8 × 106 cells/kg recipient weight respectively.Conclusion: FCM is a rapid convenient method for detectingCD34+ Cells in allo-PBSCT.
出处
《兰州医学院学报》
1998年第1期32-33,共2页
Journal of Lanzhou Medical College