丹参素异丙酯对大鼠肺动脉的舒张作用及机制

Relaxant effect of salianic acid A isopropyl ester on rat pulmonary artery and its mechanism

目的观察丹参素异丙酯的舒张血管作用,并探讨其作用机制。方法以大鼠肺动脉为标本,采用离体血管灌流方法,观察丹参素异丙酯对去甲肾上腺素(NE)预收缩动脉的舒张作用以及血管内皮细胞、血管平滑肌在该效应中的作用。结果丹参素异丙酯可浓度依赖性舒张NE预收缩的完整内皮血管,去内皮后该作用明显降低。可使KCl量效曲线右移,并能浓度依赖地抑制NE诱导的内钙释放和经受体操纵性钙通道(ROCC)的外钙内流。L-Nω-硝基精氨酸甲酯(L-NAME)、吲哚美辛以及CsCl、四乙铵、格列本脲和BaCl2均可显著减弱丹参素异丙酯的舒血管作用,而普萘洛尔、美托洛尔及沙丁胺醇对丹参素异丙酯的舒血管效应无明显影响。结论丹参素异丙酯可内皮依赖性地舒张大鼠肺动脉,其舒张血管效应可能与NO、前列环素(PGI2)途径以及钙通道、钾通道有关。

Objective To investigate the relaxant effect of salanic acid A is：opropyl ester （isopropyl 3- （3,4-dihydroxyphenyl）-2-hydroxypropanoate） and its mechanism. Methods Isolated rat pulmonary arterywas perfused and the tension of the vessel was measured. To observe the relaxant effect of salianic acid A isopropyl ester on the pulmonary artery precontracted by noradrenaline （NE） and the role of endothelial cell and vascular smooth muscle cell on vasodilation. Results Salianic acid A isopropyl ester relaxed the endothelium-intact artery precontracted by NE in a concentration-dependent manner, and the effect was de- pressed with denuded endothelium. Salianic acid A isopropyl ester right-shifted the concentration-response curve of KC1 and suppressed the contractions induced by NE and CaCl2 in the Ca2＋-free-K-H solution. The relaxant effect of salianic acid A isopropyl ester was inhibited by N-nitro-L-arginine methyl ester hydro- chloride （L-NAME）, Indometacin, CsCl, Tetraethylammonium （TEA）, Glibenclamide, and BaCl2, but not that by Propranolol, Metoprolol, and Salbutamol. Conclusion Salranic acid A isopropyl ester could significantly relax the pulmonary artery of rats, which may derive in part from endothelium. The relaxant effect of salianic acid A ispropyl ester may relate to NO pathway, prostacyclin （PGI2） pathway, calcium channel, and potassium channel.