野西瓜挥发油抑制人胃癌SGC-7901细胞增殖的研究

Inhibition of volatile oil in Capparis spinosa on proliferation of human gastric carcinoma SGC-7901 cells

目的研究野西瓜挥发油的抗肿瘤作用。方法采用MTT实验和SRB实验观察野西瓜挥发油对SGC-7901细胞的体外抗肿瘤作用;采用倒置荧光显微镜观察野西瓜挥发油作用后典型的细胞凋亡形态;采用流式细胞仪测定野西瓜挥发油对SGC-7901细胞凋亡及细胞周期的影响。结果10～200μg/mL的野西瓜挥发油作用于SGC-7901细胞72h后均可不同程度的抑制SGC-7901细胞的增殖,其IC50为145.5μg/mL,GI50为121.32μg/mL,LC50为900.96μg/mL,TGI为240.55μg/mL;作用24h后出现典型的凋亡形态;75、150、300μg/mL的野西瓜挥发油作用24h时出现细胞凋亡;周期分布发生改变,呈现S期阻滞。结论野西瓜挥发油具有明显抑制SGC-7901细胞增殖作用,其SGC-7901机制为诱导肿瘤细胞凋亡及改变细胞周期。

Objective To study the anti-tummor effect of the volatile oil in Capparis spinosa. Methods MTT and SRB were used to study the anti-tummor effect of the volatile oil in vitro. The typical morpholo- gy of apoptosis induced by the volatile oil in C. spinosa was abserved by reversed fluorescent microscope. The effect of the volatile oil on cell apoptosis and different stages of cell cycles in human gastric carcinoma SGC-7901 cells was studied by flow cytometer. Results The proliferation of SGC-7901 cells was inhibited after treated with the volatile oil （10, 50, 100, 150, and 200 μg/mL） for 72 h and the ICs0 was 145.5 μg/mL, GI50 was 121.32 μg/mL,LC50 was 900.96 μg/mL, TGI was 240.55 μg/mL. After SGC-7901 cells were treated by the volatile oil for 24 h, the typical morphological changes of apoptosis were observed, the apoptotic cells were detected after treated with the volatile oil （75, 150, and 300 μg/mL） for 24 h. Cell cycle was also analyzed, with the results showing that the volatile oil led S stage block. Conclusion The volatile oil has the significant inhibition on proliferation of SGC-7901 cells. Its anti-tummor mechanism could be attributed to its action of initiating the apoptosis of tumor cells.