脉冲场凝胶电泳分析耐甲氧西林金黄色葡萄球菌

Analysis of methicillin resistant Staphylococcus aureus with pulsed field gel electrophoresis

目的探索一种准确、快速、可靠的分子生物学分型方法，研究耐甲氧西林的金黄色葡萄球菌（ＭＲＳＡ）的流行病学。方法建立脉冲场凝胶电泳（ＰＦＧＥ）的分子生物学分型方法。将细菌包埋于琼脂块中，原位溶解细菌，ＳｍａⅠ消化染色体ＤＮＡ，经脉冲场凝胶电泳（ＰＦＧＥ）分离，比较染色体限制性内切酶图谱，确定菌株的亲缘关系。结果３８株ＭＲＳＡ的ＰＦＧＥ谱分１０组（Ａ～Ｊ），以Ａ型为主，Ａ型又有Ａ１～Ａ４四个亚型。１９９５年１１月～１９９６年３月发生了Ａ１亚型株暴发流行，从１８位住院病人共分离到２２株。暴发流行株在住院病人、各病房及各医院之间进行传播。结论对ＭＲＳＡ的流行病学进行研究，ＰＦＧＥ是一种准确、可靠、重复性好的分子生物学分型方法。

Objective To explore the rapid,accurate and dependable procedures for invastigating the epidemiology of methicillin resistant Staphylococcus aureus (MRSA).Methods A method of using pulsed field gel electrophoresis (PFGE) was established for typing the DNA of MRSA.Cells were suspended in agarose and cells were digested in situ.The intact genomic DNA was cleaved with SmaⅠ and electrophoresised in puled field. Results Ten different PFGE patterns(A J) were found among the 38 MRSA strains,most of MRSA with PFGE type A. 22 MRSA strains with PFGE type A1 were isolated from 18 inpatients spreading over a long time period during the outbreak (from Nov.1995 to Mar.1996).The outbreak strain was spread over a number of patients,hospital wards and different hospitals. Conclusion Invastigating the epidemiology of MRSA,PFGE is an accuratd,accessible and useful genotyping method.