麝香草酚体外抗真菌活性研究

Study on in Vitro Antifungal Activity of Thymol

白色念珠菌等真菌是人类及动物重要致病菌。测定麝香草酚(THY)对临床分离红色毛癣BMU01672株、酿酒酵母标准株和24株临床分离耐氟康唑(FLC)白色念珠菌的最小抑菌浓度(MIC),采用96孔平板微量法测定了THY/FLC,THY/AMB(两性霉素B)联合用药的分级抑制浓度指数(ractional inhibitory concentrationindex,FICI)值。结果表明,THY有较好的抗真菌活性,THY与FLC和AMB联合应用具有协同抗真菌作用,这为麝香草酚抗真菌活性的进一步研究和开发打下了基础。

Candida albicans （C. albicans） and other fungi are important human and veterinary pathogens. At present, the application of effective clinical drugs are limited, because the drugs have high toxicity and are easy to have a resistance that are causing the rate of relapse and re-infection rate significantly increased and a number of adverse reactions. There was an urgent need to develop high-performance, low toxicity of new drug against resistant fungi. The minimal inhibitory concentration of thymol （THY） tested against clinical trichophyton rubrum strain BMU 01672, Saccharomyces cerevisiae standard strain and 24 clinical C. albicans, and checkerboard assay was used to analyzed the factional inhibitory concentration index （FICI） of THY/FLC, THY/AMB. The result showed that THY had good antifungal activity, and THY/FLC and THY/ AMB showed synergism in antifungal interaction.