SSR标记应用于烟草品种遗传多样性研究

Genetic Diversity Revealed by SSR Markers of the Tobacco Cultivars

SSR标记是进行植物遗传多样性研究的理想技术。研究应用2007年Bindler等[14]公布的的烟草SSR标记分析了13个云南省烟草主栽品种的遗传多样性。通过对92对分布于烟草24个连锁群的SSR标记引物筛选,发现20对在这些品种之间存在多态性。20个SSR位点上共检测到52个等位基因,平均每对引物等位基因数为2.6个。根据SSR标记多态性计算了不同品种之间的遗传距离(GD),13个品种之间遗传距离介于0.0090～0.4286之间,平均距离为0.2237,说明品种间遗传差异较小。SSR标记技术将在烟草的基因标记定位及分子辅助育种中发挥重要的作用。

SSR marker is the ideal techniques for analysis of plant genetic diversity. In this study the genetic diversity among 13 main cultivars in Yunnan province which published recently. 92 SSR located on 24 tobacco were measured using these tobacco SSR markers linkage groups were screened, among which 20 were polymorphic. Fifty-two alleles were amplified in 13 cuhivars using these 20 SSR primer pairs, with 2.6 alleles per primer pair. The genetic distance （GD） in 13 tobacco cuhivars was calculated based on the SSR marker polymorphism, ranging from 0.0090-0.4286, with the average of 0.2237. This result indicates the genetic variation of these cuhivars were very little. Tobacco SSR markers can be used to analyze effectively the genetic diversity of tobacco germplasms and of great value for molecular genetic mapping and marker assisted selection in tobacco breeding.