蝴蝶兰^(60)Coγ射线辐照材料的RAPD分子标记研究

Study on the Radiation of the ～(60)Coγ-ray for Variation Induction in Phalaenopsis aphrodite by Using of RAPD-PCR Technique

为获得V31蝴蝶兰的最佳辐射诱变剂量,利用60Coγ射线对其组培盆栽苗进行不同剂量的辐照处理,取辐射处理苗新叶提取g-DNA,应用RAPD-PCR技术进行扩增。试验结果表明,利用从40个随机中筛选出的13条引物,共扩增出60条谱带,其中特异性条带26条。对不同处理及对照扩增的谱带进行聚类分析发现,15Gy处理整体变异程度最大,1Gy变异最小。因此,认为V31蝴蝶兰的最佳辐射诱变剂量为15Gy。

In order to get the best radiation dose of the ^60Coγ-ray for variation induction in Phalaenopsis aphrodite cv. V31, the potted clonal plants were treated by different doses of ^60Coγ-ray. The genomic DNA of treated and controlled plants was extracted from young leaf and amplified by using of RAPD-PCR technique. The 60 bands were amplified by using the 13 primers screened out from 40 ones and 26 bands of that were special. The cluster analysis of the bands amplified in treated and controlled plants showed that the variation degree was maximum in plants treated with 15Gy and was minimum in plants treated with 1Gy. Therefore 15Gy was the best radiation dose of the ^60Coγ-ray for variation induction.