苹果轮纹病病菌分生孢子离体人工诱导技术研究

Studied on Techniques of Conidiophores inducing Culture in vitro in Apple Ring Spot

为进一步了解苹果轮纹病菌的生物学特性,解决其在离体培养条件下产生分生孢子很少的问题,研究了培养基pH、培养温度、光源和光照条件对菌丝体生长和分生孢子发生的影响以及刮除菌丝体对分生孢子发生的影响。结果表明,24～28℃较适宜菌丝体生长,24℃时分生孢子囊和分生孢子发生最多;培养基pH5.0以及接种后在黑光灯下培养有利于分生孢子囊和分生孢子产生;菌丝体生长到一定程度后,刮除菌丝体对分生孢子囊和分生孢子的发生有显著促进作用。

In order to understand the biology of apple ring spot fungi further and solve the problem of less conidiophores generated during their inducing culture in vitro, it was studied that the effect of culture medium pH, incubation temperature, light sources, and light regiment on growth of mycelia and generation of conidiophores, and the removing of mycelia on generation of conidiophores. The results showed that mycelia grew well under 24-28℃ condition, and could generated conidiophores most. Adjusted culture medium pH to 5.0 and incubated under black light after inoculation could benefit to the conidiophore＇s generation. The removing of mycelia after their growth to some extent had significant promoting effect on the conidiophore＇s generation.