外源蛋白酶对蚯蚓蛋白酶解的影响

The Effect of Outer Proteases on the Enzymatic Hydrolyze of Earthworm Protein Pulp

蚯蚓为多功能性生物资源,利用酶解工艺将蚯蚓蛋白制作成的氨基酸原料有广泛的用途,为进一步提高蚯蚓蛋白水解效率;该文在实验室条件下研究了三种外源酶对蚯蚓(Eiseniafetida)粗蛋白下脚料水解的影响,重点探讨了酸性蛋白酶加入后鲜蚯蚓浆水解过程中氨基酸浓度的变化,建立了水解回归方程;结果表明:三种外源蛋白酶对蚯蚓粗蛋白水解率的影响明显,与对照不加酶相比均提高了水解率,顺序为酸性蛋白酶>碱性蛋白酶>中性蛋白酶。鲜蚯蚓浆水解产物的氨基酸浓度与水解时间存在明显的相关性,加酶处理与不加酶处理的回归方程分别为:Y=0.432371*EXP(0.130930*X);Y=0.260455*EXP(0.143428*X),回归方程均达到显著水平。与不加酶处理相比,酸性蛋白酶处理显著提高了鲜蚯蚓浆水解过程中的氨基酸浓度。水解提高率随着水解时间的延长而下降,提高率与水解时间两者呈负相关;可以得出酸性蛋白酶对蚯蚓浆的水解率提高明显,提高了生产速率,为下一步开发以蚯蚓氨基酸为基础的多功能液体肥料提供参考。

Earthworm is a versatile bio-resource and the hydrolysis products of its protein are a useful material. In order to increase the hydrolysis efficiency; acid protease was used to the hydrolysis of earthworm protein pulp and three outer proteases were used to the hydrolysis of earthworm protein trash. The effect of acid protease on the amino acid concentration for earthworm protein pulp was mainly studied in this paper. Based on the relationship of hydrolysis time and amino acid concentration a regress equation model was developed; The result showed that outer protease had significant effect on hydrolysis of protein trash. The hydrolysis rate of 3 treatment that had outer proteases was increased compared with CK, the sequence was： acid protease〉alkaline protease〉neutral protease. The regression approach equations for the hydrolysis time and amino acid concentration were： Y=0.432371＊EXP （0.130930＊X） for acid protease treatment; Y=0.260455＊EXP （0.143428＊X） for CK. The significant test for the equations showed that all got a significant level. The amino acid concentration was increased significantly for the acid protease treatment. The increasing rate of hydrolysis was decreased with the increase of hydrolysis time, and had a negative correlation during hydrolysis process; In conclusion, outer acid protease is a significant effects on hydrolysis of earthworm, improved rate of production, which supplied a new way to empolder function liquid fertilizer ,based on animo acid from earthworm hydrolyte.