IL-2基因直接转移促进CD4^+缺损小鼠免疫功能的恢复

Recovery of the immune function in CD4 + deficient mice by IL 2 gene transfer

目的探讨ＩＬ－２基因直接转移治疗小鼠ＣＤ４缺陷的可能性。方法ｐＣＩ－ｈＩＬ－２质粒ＤＮＡ直接注入ＣＤ４缺陷小鼠大腿肌肉内。用免疫组化法检测ｈＩＬ－２蛋白的表达，同时测定小鼠的免疫功能。结果ｐＣＩ－ＩＬ－２质粒ＤＮＡ可在注射部位肌纤维内表达ＩＬ－２蛋白。ＣＤ４缺陷小鼠脾细胞中ＣＤ４＋Ｔ细胞增多，脾细胞对有丝分裂素ＣｏｎＡ增殖反应明显增强。对ｓＲＢＣ的免疫应答无论是体液免疫或细胞免疫应答均高于对照组（Ｐ＜０．０１）。结论ｐＣＩ－ＩＬ－２质粒ＤＮＡ直接肌内注射可部分纠正ＣＤ４缺陷小鼠的免疫功能。

Objective To explore the possibility of correcting the immunodeficiency of CD4 deficient mice through IL 2 gene direct transfer. Method pCI hIL 2 plasmid DNA was injected into muscle of CD4 deficient mice.The mice injected with pCI IL 2 plasmid DNA were examined immune function and expression of IL 2 protein.Result (1)The result of immunohistochemistry suggested that myofiber had expressed the hIL 2 protein.(2) Injection of pCI IL 2 plasmid DNA result in an increased number of CD4 + T cell and enhanced the proliferation response of splenocytes to Con A.(3)The immune response of mice injected with pCI hIL 2 plasmid DNA to sRBC are much higher than the control mice. Conclusion All the results indicated that the expressed hIL 2 in muscle cell after direct DNA injection could partly correct the immunodeficiency of CD4 deficient mice.