gp130单克隆抗体(B-K5)抑制OM的生物学活性

Anti gp130 monoclonal antibody B K5 inhibited principally the biological activity of OM

目的一组抗ｇｐ１３０分子的单克隆抗体已被制备。对这组单抗的生物学及功能特性的研究首次表明，ｇｐ１３０分子上不同位点涉及不同的ｇｐ１３０细胞因子的生物学活性。在本研究中，其中一个抗ｇｐ１３０单抗，Ｂ－Ｋ５，将被作进一步研究。方法采用ｇｐ１３０细胞因子ＯＭ和ＬＩＦ，Ｂ－Ｋ５单抗以及对ｇｐ１３０细胞因子高度敏感的细胞株（ＸＧ４－ＣＮＴＦ），通过３Ｈ－ＴｄＲ的掺入作生物学检测，以期进一步确定Ｂ－Ｋ５单抗的生物学特性。结果当ＯＭ和ＬＩＦ的浓度各自为３００ｐｇ／ｍｌ和１００ｐｇ／ｍｌ时，Ｂ－Ｋ５单抗可抑制ＯＭ和ＬＩＦ的生物学活性；但当ＯＭ的浓度上升至３ｎｇ／ｍｌ时，Ｂ－Ｋ５仍可抑制ＯＭ的生物学活性。相反，当ＬＩＦ浓度在１ｎｇ／ｍｌ时，Ｂ－Ｋ５却不能抑制了，即使Ｂ－Ｋ５单抗的浓度已被提高。结论Ｂ－Ｋ５单抗主要影响ＯＭ的生物学活性。

Objective A group of anti gp130 monoclonal antibodies (McAbs) was prepared.The biological and functional characterization of these McAbs has found for the first time that the different epitopes on gp130 molecule were involved in different biological activities of gp130 cytokines.One anti gp130 McAb,B K5,will further be characterized in this study.\ Methods\ [WT5”BZ〗The gp130 cytokines OM and LIF,B K5 McAb. and a cell line(XG4 CNTF)highly sensitive to gp130 cytokines were used for biological assay by virtue of 3 H TdR incorporation in order to further determine biological characteristics of B K5 McAb.\ [WT5”HZ〗Results\ B K5 McAb inhibited the biological activities of OM and LIF when OM and LIF concentrations were 300pg/ml and 100pg/ml, respectively.But,B K5 could still inhibit the biological activity of OM when concentration of OM was increased to 3ng/ml,whereas B K5 could not while LIF was at the concentration of 1ng/ml even if concentration of B K5 was enhanced. \ [WT5”HZ〗Conclusion\ B K5 McAb affected mainly the biological activity of OM.