摘要
用体外原代培养的人脐静脉内皮细胞（ＨＵＶＥＣ）观察ｒＴＮＦα、ｒＩＬ－１对ＨＵＶＥＣ产生ＩＬ－１的诱导效应及重组ＩＬ－１受体拮抗剂（ｒＩＬ－１ｒａ）的抑制效应。结果表明，ｒＴＮＦα以剂量依赖的方式促进ＨＵＶＥＣ释放ＩＬ－１；刺激８ｈＩＬ－１的释放活性显著提高，２４ｈ达峰值。ｒＩＬ－１也可促进ＨＵＶＥＣ释放ＩＬ－１，随ｒＩＬ－１浓度的增加，ＩＬ－１的释放活性也增加，刺激４ｈ，ＩＬ－１的释放活性即可明显提高（Ｐ＜０．０１）。ｒＩＬ－１ｒａ可抑制由ｒＩＬ－１引起的ＩＬ－１释放，提高ｒＩＬ－１ｒａ的浓度，上清液中ＩＬ－１活性相应减低，当ｒＩＬ－１ｒａ浓度达１０ｍｇ／Ｌ时。
The studies were made on the effect of production of IL 1 in HUVEC induced by rTNFα,rIL 1 and the inhibitory effect of rIL 1ra.The results showed that rTNFα promoted HUVEC to release IL 1 in a dose dependent manner.When HUVEC was stimulated,the secretion of IL 1 rose significantly 8 hours later and reached peak 24 hours later.Recombined IL 1(rIL 1)promoted HUVEC to produce IL 1.The production of IL 1 rose in proportion to rIL 1 dose dependently.The activity of release of IL 1 rose obviously 4 hours after HUVEC was stimulated( P <0.01).rIL 1ra inhibited IL 1 secretion of HUVEC induced by rIL 1.Activity of IL 1 in supernatant decreased when the concentration of rIL 1ra increased at the same time.When rIL 1ra reached 10mg/L.activity of IL 1 could be inhibited almost completely.
出处
《山东医科大学学报》
1998年第2期107-111,共5页
Acta Academiae Medicinae Shandong
基金
山东省自然科学基金会青年科学基金
关键词
脐静脉
内皮细胞
白细胞介素1
受体
拮抗剂
Umbilical veins
Endothelium cells
Interleukin 1
Interleukin 1 receptor antagonist
