南京地区人群CYP2E1基因多态性及吸烟、饮酒与前列腺癌易感性

Correlation of Prostate Cancer Susceptibility with Genetic Polymorphism of Cytochrome P450 2E1, Smoking and Drinking:A Case-Control Study in the Population of Nanjing Area

目的:探讨Ⅰ相代谢酶细胞色素P450 2E1(CYP2E1)基因RsaⅠ和PstⅠ位点多态性及吸烟、饮酒习惯与前列腺癌(PCa)发病风险的关系,并探讨基因与生活习惯在PCa发病中的联合作用。方法:采用PCR-RFLP技术检测109例原发性PCa患者及202例年龄匹配的男性非肿瘤患者外周血CYP2E1基因RsaⅠ和PstⅠ多态位点的基因型。结果:深吸烟(OR=2.29,95%CI:1.28～4.09)、重度吸烟史(OR=1.81,95%CI:1.02～3.22)等生活习惯为PCa易感因素。CYP2E1 C1/C1基因型与PCa易感性有显著相关(OR=1.71,95%CI:1.04～2.82),且与饮酒的联合作用明显与PCa易感性相关(OR=2.21,95%CI:1.06～4.59)。重度吸烟人群中,携带CYP2E1易感基因型(C1/C1)与不吸烟且携带非易感基因型(C1/C2或C2/C2)个体相比PCa的发病风险显著增高(OR=2.80,95%CI:1.20～6.56)。结论:携带CYP2E1易感基因型(C1/C1)并有烟酒嗜好者PCa的发病风险显著增高,且与烟草的暴露呈显著剂量反应关系。

Objective： To investigate the association of the risk of prostate cancer （PCa） with the polymorphism of the CYP2E1 gene, smoking and drinking, and to explore the joint role of genes and living habits in PCa pathogenesis. Methods ： We conducted a casecontrol study on 109 PCa patients and 202 age-matched non-PCa male controls, and detected the polymorphisms of CYP2E1 Rsa Ⅰ and Pst Ⅰ sites by PCR-RFLP using DNA from peripheral blood lymphoeytes. Results ： The history of deep smoking （ OR = 2.29, 95% CI： 1.28- 4.09） or heavy smoking （OR = 1.81, 95% CI： 1.02- 3.22） was a risk factor. The CYP2E1 C1/C1 genotype significantly increased the risk of PCa （OR = 1.71, 95% CI： 1.0d -2.82） and apparently interacted with drinking （OR = 2.21, 95% CI ： 1.06 - 4.59）. Heavy smokers with the C1/C1 genotype showed an increased risk of PCa （OR = 2.80, 95% CI ： 1.20 - 6.56）, as compared with non-smokers carrying the genotype of C1/C2 or C2/C2. Conclusion ： The risk of PCa obviously increases in individuals witfi both tile CYP2E1 C1/C1 genotype and the fiabit of smoking or drinking, and it has a significant positive correlation with the dose of tobacco exposure.