大鼠原位肝移植手术技巧探讨

Techniques for orthotopic liver transplantation in rats

目的:探讨建立稳定的大鼠原位肝移植模型的外科技巧。方法:在Kamada二袖套法基础上,着重对供肝分离、灌注、肝上下腔静脉切取,肝上下腔静脉吻合,袖套和胆道处理等手术方法作了进一步改进。结果:共建立40例大鼠原位肝移植模型,手术成功率80%,7 d存活率达70%。结论:在娴熟细致的外科操作基础上进一步缩短无肝期,可顺利完成大鼠肝移植模型的建立。

Objective： To investigate the surgical techniques for establishing the rat model of orthotopic liver transplantation. Methods ： On the basis of the double-cuff technique of Kamada, we improved the techniques for the separation and perfusion of the donor liver, the shearing and anastomosis of the superior and inferior caval veins, and the anastomosis of the bile duct. Results ： Of the 40 rats that underwent orthotopic liver transplantation, 80% survived longer than 24 hours and 70% over 7 days. Conclusion With extreme patience and carefulness, the operator can successfully establish the rat model of orthotopic liver transplantation by shortening the anhepatic phase with skillful surgical techniques.