依达拉奉对次声性脑损害的保护

Neuroprotective effects of edaravone on brain injuries caused by infrasound

为了观察16Hz、130dB次声对SD大鼠记忆功能的影响及依达拉奉对其脑损害的防治作用。依达拉奉干预组在次声作用前3d开始连续给药(3mg/kg)直至次声作用7d后停止。次声作用7d后,测Y型电迷宫评定大鼠记忆功能,用单链DNA(single-stranded DNA,ssDNA)免疫标记法检测海马内ssDNA阳性细胞(凋亡细胞)数,并通过透射电镜观察脑超微结构。结果发现次声作用后大鼠学习记忆功能下降、海马ssDNA阳性细胞数明显增多(与正常对照组相比,P<0.05);电镜下可见神经细胞出现较严重缺血、缺氧性改变,并可见不同时期凋亡细胞。依达拉奉干预组大鼠学习记忆成绩与空白对照组相比无差异;海马ssD-NA阳性细胞数较次声对照组明显减少,但仍较正常对照组多,差异均有统计学意义;电镜下细胞变性改变程度较轻。由此得到结论16Hz、130dB次声可引发大鼠的脑损伤、细胞凋亡、记忆功能受损,依达拉奉可明显减轻次声引发的这些损害。

To observe the protective effects of edaravone on brain injuries caused by infrasound （ 16 Hz, 130 dB）. The edaravone group was administered 3 d before infrasonic exposure and lasted for 10 d. At the end of experiment, every rat was evaluated by the method of Ymaze memory test, apoptosis was detected with ssDNA immunostaining method. The changes of uhramicrostructure were observed under transmission electron microscope. In infrasound control group, a remarkable decline of memory function and a noticeable increase in the number of ssDNA-positive ceils in hippocampus were observed, while treatment with edaravone showed recovery of impaired memory in rats exposed to infrasound, and exhibited potent anti-apoptotie activity in rat hippocampal neurons. Electron microscope images showed that infrasound induced typical hypoxia and ischemic changes in cells, which was also ameliorated by the drug. Edaravone exerts protective effects against brain injuries caused by infrasonnd, such as memory function decline, uhramierostructure injury and apoptosis.