摘要
MTT方法具有灵敏、简便、稳定可靠、不需同位素等特点。为了摸索测定昆虫细胞存活（或死亡）的MTT方法的各种最适条件，本文以中国棉铃虫细胞系为对象，确定了一些基本参数、并比较了三种MTT测定方法。结果表明：改进的方法所选用的溶剂——pH为4.5的3％SDS异丙醇效果最好，溶解甲所需时间短、OD值高。测定昆虫细胞范围为每孔500～60000细胞，测试波长为560nm，参考波长为690nm.
In order to optimize a rapid, sensitive and simple colorimetric insect cell viability assny (MTT assay),three methods have been tested for their ability to solubize formanzan, It is found that the solvont of the modified method, pH4.5, 3% SDS-isopropand, is superior to the others. All samplos were measuned with ELISA scanner at wavelength 560nm for measuernent and 690nm for reference, Th linearity can be obtained at insect cell numbers within the range of 500-60000 cells per well.
出处
《昆虫知识》
CSCD
1998年第3期165-167,159,共4页
Entomological Knowledge
基金
湖北省科委重点科技研究项目
