火炬松原生质体的体细胞胚胎发生

SOMATIC EMBRYOGENESIS FROM PROTOPLASTS OF LOBLOLLY PINE

研究了基本培养基、原生质体密度和ABA浓度对火炬松（PinustaedaL．）悬浮细胞原生质体体细胞胚胎发生的影响。结果表明，DCR基本培养基最有利于原生质体的体细胞胚胎发生。体细胞胚胎发生所需的最适原生质体密度和ABA浓度分别是7×104个／mL和4mg／L。显微观察表明，来自原生质体的胚性胚柄细胞团（ESM：embryogenicsusPensormass），经早期原胚（ESP：earlystageProembryos）阶段形成了后期原胚（LSP：latestageProembryos）。这一结果为火炬松的原生质体培养再生植株奠定了基础。

Influences of basic media,protoplast density,and ABA concentration on somatic embryogenesis of loblolly pine were studied. The results show that DCR medium is in favor of the formation of somatic embryos from protoplasts. And the optimum protoplast density and ABA concentration for somatic embryogenesis are 7 ×104/mL and4 mg/L, respectively. The microscopical observation showed that embryonal suspensor masses developed firstly into early stage proembryos,then into late stage proembryos.These results lay the foundation of plantlet regeneration from protoplast of loblollypine.