马尾松染色体荧光带型的研究

STUDY ON CHROMOSOME FLUORESCENT BANDING PATTERN IN PINUS MASSONIANA

对马尾松有丝分裂中期染色体荧光带纹的分析结果表明，其色霉素A的染色体的荧光带型为：1对为着丝粒区和臂间区均有带纹的中间着丝粒染色体；6对为臂间区有带纹的中间着丝粒染色体；1对为着丝粒区有带纹的中间着丝粒染色体；3对无带纹的中间或近中着丝粒染色体；1对为着丝粒区有带纹的近中着丝粒染色体。而DAPI的染色体的荧光带型为：3对为着丝粒和臂间区均有带纹的中间着丝粒染色体；1对在臂间区有带纹的中间着丝粒染色体；6对为着丝粒区有带纹的中间着丝粒染色体；2对无带纹的中间或近中着丝粒染色体。最后对荧光方法的实验方法和应用进行了讨论。

Root-tip metaphase chromosomes of Pinus massoniana were sequentiallystained with CMA and DAPI. P. massoniana had a pair of metacentric chromosomes withtwo CMA-bands at proximal and interstitial regions,six pairs of metacentric chromosome with interstitial CMA-band, a pair of metacentric chromosome with proximalCMA-band, and three pairs of metacentric or submetacentric chromosome withoutCMA-band as well as a pair of submetacentric chromosome with proximal CMA-band.But the DAPI banding pattern was different from CMA banding pattern. P. masonianahad three pairs of metacentric chromosomes with two DAPI-bands at proximal and interstitial regions, a pair of metacentric chromosome with interstitial DAPI-band, sixpairs of metacentric chromosome with proximal DAPI-band,and two pairs of metacentric or submetacentric chromosome without DAPLband.