摘要
初步研究了大丽轮枝菌（ＶｅｒｔｉｃｉｌｉｕｍｄａｈｌｉａｅＫｌｅｂ．）硝酸盐利用缺陷型和抗杀菌剂突变体的遗传特征。结果表明，大丽轮枝菌对三环唑的抗性不能稳定遗传，抗性菌株经低温（４℃）或室温保存一个月及转代培养后均丧失抗性，５株抗多菌灵的突变体，有一株在第二代单孢后代培养时丧失抗性，１株在单孢后代中发生分离；１０株ｎｉｔ突变体经保存３个月后，有一株恢复成野生型。其余突变体经转代培养、低温或室温下保存一年及接种棉苗后仍都保持原有的培养性状。ｎｉｔ突变体对棉苗的致病力普遍降低；
The inheritance of pathogenicity and culture character of the mutants in Verticillium dahliae were tested. The results indicated that all of five tricyclazole-tolerant isolates of the fungus lost resistance after storage and successive mass transfers; one out of ten nitrate-tonutilizing (nit) mutants reversed to wild-type after three months storage, but others were stable and caused lower infection rate than their wild-type isolates. One of five carbendazim resistant mutants lost resistance on second single spore generation and the resistance segregation occurred in one of them. There was no significant change in pathogenicity between carbendazim-sensitive and resistant isolates. Reaquired mutant phenotypes of both nit and carbendazim-resistant mutants showed the stable cultural characteristics after inoculating cotton.
出处
《菌物系统》
CSCD
北大核心
1998年第2期137-142,共6页
Mycosystema
基金
新疆农业大学校长基金
江苏省"九五"攻关课题资助
关键词
大丽轮枝菌
抗杀菌剂
突变体
遗传
erticillium dahliae, nit mutant, Fungicide-resistant mutant, Inheritance.
