摘要
耐温的克鲁维酵母（Ｙ０３４）与产酒率高的酿酒酵母（Ａ００１）进行属间原生质体融合构建耐温酵母菌株，经ＤＴＴ分段预处理获得大量具再生活性原生质体对融合株ＡＹ０２３等进行了乙醇脱氢酶同工酶，麦芽糖同化，遗传稳定性及高温发酵分析，融合株ＡＹ０２３表达了双亲遗传特性。在４５℃高温发酵条件，乙醇产率高达７．４％。
An ethanol-producing and thermotolerant yeast strain was constructed by protoplast fusion. The fusant AY 023 appears to possess features of the parent strains, such as alcohol dehydrogenase pattern, maltose assimilation and so on. It can ferment glucose into ethanol at 45℃ and ethanol production is up to 7.4%. This is the first report about high temperature fermentation (45℃) by yeast fused by protoplast fusion.
出处
《菌物系统》
CSCD
北大核心
1998年第2期143-147,共5页
Mycosystema
关键词
耐温性能
克鲁维酵母
酿酒酵母
原生质体融合
Thermotolerance, Saccharomyces cerevisiae, Kluyveromyces, Protoplast fusion
