用做标记的两种酵母菌完整染色体DNA的简化制备法

SIMPLIFIED PREPARATION METHOD OF TWO YEASTS MOLECULAR MARKERS FOR PULSED FIELD GEL ELECTROPHORESIS

已知分子量大小的染色体ＤＮＡ分子标记是脉冲电泳研究中用以估算样本染色体ＤＮＡ分子大小必不可少的参照物。对用做标记的啤酒酵母（Ｓａｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ）和粟酒裂殖酵母（Ｓｃｈｉｚｏｓａｃｈａｒｏｍｙｃｅｓｐｏｍｂｅ）完整染色体ＤＮＡ几种制备方法的比较研究以及改进研究表明，液氮冷冻研磨法，凝胶包埋法以及凝胶包埋破壁法效果均很好，都得到大量染色体ＤＮＡ，且彼此间无明显差异。表明液氮冷冻研磨法和凝胶包埋法制备上述两种酵母菌染色体ＤＮＡ分子标记是两种理想的方法，可完全取代凝胶包埋破壁法，即缩短处理时间又降低成本。此外，相同的电泳样本块在不同的电泳条件下，所得结果有一定的差异。

Several simplified preparation methods for Saccharomyces cerevisiae and Schizosaccharomyces pombe molecular markers were comparatively studied. All the liquid nitrogen freeze-grind method, the agarose-embedding method and the agarose-embedding-lysis cell wall method gave good results. Moreover, there was no remarkable difference in chromosomal DNA content and chromosomal number between these three methods. The liquid nitrogen freeze-grind method and agarose-embedding method may replace the agarose-embedding-lysis cell wall method.