摘要
利用反转录ＰＣＲ（ＲＴＰＣＲ）法从人肝组织中分离人白介素１８（ｈＩＬ１８）基因。克隆到Ｔｖｅｃｔｏｒｅａｓｙ载体中，经酶切初步鉴定后进行ＤＮＡ序列分析，结果表明与文献报道完全一致。以ｐＢＶ２２０为表达载体，在大肠杆菌中高效表达了ｈＩＬ１８基因，重组蛋白占菌体总蛋白的２７．２％。为进一步研究其生物活性奠定了重要基础。
Human interleukin 18 gene was isolated from human liver tissue by reverse transcription polymerase chain reaction.hIL 18 gene was cloned into plasmid pGEM T(Easy)and pBV220.The gene was sequenced and showed no difference with that reported and expresed in E.coli in the form of inclusion bodies.The recombinant protein amounts to 27.2% of total bacterial protein.
出处
《中国病毒学》
CSCD
1998年第2期134-138,共5页
Virologica Sinica
基金
河北省科委资助
