生物素标记寡核苷酸探针检测B群轮状病毒

Detection of the Group B Rotavirus with the Probe of Oligonucleotides Labelled with Biotin

用５′端接氨基标记法，用生物素对Ｂ群轮状病毒（ＧＢＲＶ）ＩＤＩＲ株具有群特异性的３片段基因上２３ｂｐ寡核苷酸序列进行标记，经高效薄层层析板（ＨＰＴＬＣ）纯化，制备了Ｂ群轮状病毒生物素寡核苷酸探针，探针显色灵敏度达１０Ｐｇ，与ＧＢＲＶ、ＫＢ６３株基因杂交可检测到１００ｐｇ靶序列，而与Ａ群和Ｃ群轮状病毒及无关基因均不产生杂交信号，该探针可用于Ｂ群轮状病毒的检测、鉴定，以及同群不同毒株间基因相关性研究。

According to segment 3 sequence of group B rotavirus (GBRV)IDIR strain the 23 bp oligonucleotide was synthesized and labelled with biotin.The probe was purified by HPTLC.Its sensitivity is up to 10pg by a colorimetric reaction.The probe can detect at least 100pg target seuqence of GBRV and does not hybridize to Group A and Group C rotavirus.The results showed that the probe has high specificity and sensitivity.It can be used to detect and identify GBRV,and also to research the genetic relativity of GBRV gene mutation and rearrangement.