摘要
应用放射性标记、薄层层析和阴离子交换柱层析技术,证明黄瓜(CucumissativaL.)细胞中存在肌醇脂质信使系统,并且水杨酸(SA)能够促进肌醇磷脂代谢,激活磷脂酶C(PLC),促进磷脂酰肌醇降解,导致第二信使肌醇1,4,5三磷酸(IP3)和双酰甘油(DAG)含量增加,表明SA信号传递有可能通过肌醇脂质信使系统的介导来完成。SA的生理功能尤其在诱导植物抗病性中的作用很可能是通过肌醇脂质信使系统介导的。
For the first time, the signal transduction pathway of salicylic acid (SA) was investigated by using 3Hlabelling, thinlayer chromatography and anion exchange column chromatography. It was found that SA stimulated the activity of membrane bound phospholipase C (PLC), accelerated the breakdown of phosphatidylinositol4monophosphate (PIP) and phosphatidylinositol4,5bisphosphate (PIP2) and increased the levels of inositol1,4bisphosphate (IP2),inositol1, 4,5trisphosphate (IP3) and diacylglycerol (DAG). These indicated that signal transduction of SA was probably accomplished through the mediation of phosphatidylinositide signal transduction system in cucumber(Cucumis sativa L.).
基金
国家自然科学基金
山东省自然科学基金
关键词
水杨酸
信号传递
肌醇磷脂代谢
黄瓜
Salicylic acid, Signal transduction, Phosphoinositide metabolism, Cucumber