哮喘小鼠肺组织中趋化因子干扰素-γ诱导蛋白-10、干扰素-γ诱导单核细胞因子表达及其意义

Expressions of IFN-γ-inducible protein-10 and monokine induced by IFN-γ of lung tissue in murine asthmatic model and its significance

目的:探讨趋化因子干扰素-γ诱导蛋白-10(IP-10)、干扰素-γ诱导单核细胞因子(Mig)在哮喘小鼠肺组织中的表达及其意义,观察地塞米松(DX)、卡介苗(BCG)干预对哮喘小鼠表达IP-10、Mig的影响。方法:健康雄性昆明小鼠40只,分为哮喘组、DX组、BCG组和对照组,每组10只。哮喘组分别于实验第1、3、5、7、9、11、13天腹腔注射卵清蛋白(OVA)10μg致敏,第21天起予1%OVA5 ml雾化吸入30 min,每天1次,连续7天,激发哮喘。DX组在雾化吸入前30 min,按2 mg/kg腹腔注射DX溶液。BCG组在致敏前7、3、1天分别皮内注射BCG 0.025 mg。对照组用生理盐水代替OVA。各组小鼠于末次雾化吸入24 h后,制备肺组织标本,免疫组化法检测肺组织中IP-10、Mig表达。结果:哮喘组IP-10表达较对照组明显增加(P<0.01),DX组明显降低(P<0.05),BCG组IP-10表达有下降,但差异均无统计学意义(P>0.05)。哮喘组Mig表达较对照组明显降低(P<0.01),DX组和BCG组增加(P<0.05)。结论:在哮喘小鼠肺组织中,IP-10表达增加,Mig表达降低;DX可以下调IP-10、上调Mig的表达;BCG上调Mig的表达,但对IP-10作用不明显。趋化因子IP-10、Mig参与哮喘发病过程,DX与BCG可不同程度干预IP-10、Mig的表达。

Objective：To evaluate the expressions and significance of IFN-γ-inducible protein-10（IP-10） and monokine induced by IFN-γ （Mig） of lung tissue in murine asthmatic model,and to observe the influence of dexamethasone（DX） and bacillus of Calmette and Guerin vaccine （BCG）on the expressions of IP-10 and Mig. Methods： Forty Kunming mice were randomly divided into asthmatic group,DX group, BCG group and control group with 10 in each. The mice in asthmatic group were sensitized and challenged with ovalbumin （OVA） by abdomen injection every other day for 13 days and atomization inhalation of 1% OVA 5 ml from d21 for 7 days to establish asthmatic models. The mice in DX group were administrated DX （2 mg/kg） by abdomen injection 30 min before challenge, the mice in BCG group were injected intradefinally with BCG at day 7,3 and 1 before sensitization and the control group were given normal saline instead of OVA. The expressions of IP-10 and Mig protein of lung tissue in the mice were detected by immunohistochemistry. Results：The IP-10 protein expression in the asthmatic group was significantly higher than that in the control group （ P 〈 0.01 ） but decreased in DX group （ P 〈 0.05 ）, and no significant decrement was observed in BCG group （ P 〉 0.05 ）. The Mig protein expression in the asthmatic group was significantly lower than that in the control group （ P 〈 0.01 ）, and the Mig protein expression in DX group and BCG group were significantly higher than that in asthmatic group （ P 〈 0. 05 ）. Conclusions： The expressions of IP-10 of lung tissue increased,but Mig decreased in the asthmatic model mice. Dexamethasone may downregulate IP-10 protein expression of lung and upregulate Mig protein expression. BCG may upregulate Mig protein expression of lung, but does not inhibit IP-10 protein expression. The chemokine IP-10 and Mig play an important role in the pathogenesis of asthma. DX and BCG can interfere with the expression of IP-10 and Mig to some extent.