四逆汤对大鼠心肺复苏后脑组织损伤的影响

Effect of Sini decoction on cerebral injury after cardiopulmonary resuscitation in rats

目的:观察四逆汤对大鼠心肺复苏(CPR)后脑组织中乳酸脱氢酶(LDH)活力和丙二醛(MDA)含量变化的影响。方法:将Wistar大鼠随机分为假手术组(C组)、心肺复苏组(CPR组)、四逆汤组(SND组)。大鼠颈外静脉内快速注入2%氯化钾,致呼吸、心跳骤停,6 min后复苏;60 min后,取大脑皮层制备10%组织匀浆,测LDH活力、MDA含量。C组动物术前用生理盐水(0.1 ml/100 g)灌胃3天后,仅完成所必要的手术操作,不做任何处理;SND组动物术前用四逆汤灌胃(0.1 ml/100 g)3天。结果:SND组脑组织匀浆LDH活力显著高于CPR组(P<0.01),MDA含量显著低于CPR组(P<0.01),但SND组的LDH和MDA与C组差异均有统计学意义(P<0.01和P<0.05)。结论:四逆汤对心肺复苏后脑细胞膜具有保护作用,可能是通过抑制氧自由基的脂质过氧化反应,稳定细胞膜来实现的。

Objective： To explore the effect of Sini decoction （SND） on the activity of lactate dehydrogenase （LDH） and the concentration of malondialdehyde （MDA） after cardiopulmonary resuscitation （CPR） in rats. Methods：Adult Wistar rats were randomly divided into 3 groups,the normal control group, the CPR group and the SND treatment group. Model of CPR was produced by injecting 2% KC1 quickly through external jugular vein. Then the rats, heartbeat and respiration stoped at once, after 6 minutes were carried out CPR. For being observed for 60 minutes, adult Wistar rats were sacrificed. The activity of LDH and the concentration of MDA in cerebral cortex were determined. 0.9% saline was fed at the dosage of 0.1 ml/100 g for three days preoperatively in the normal control group. SND was fed at the dosage of 0.1 ml/100 g for three days preoperatively in the SND group. Results：The activity of LDH in cerebral cortex was significantly higher in the SND group than in CPR group（ P 〈 0.01 ）, while the concentration of MDA was markedly lower than those in CPR group（P 〈0.01 ）. The activity of LDH in cerebral cortex were lower in the SND group than in the normal control group（P 〈0.05）, while the concentration of MDA was higher than those in the normal control group （P 〈 0.01 and P 〈 0.05 ）. Conclnsions：The results suggest that Sini decoction possiblely play a membrane protective role by suppressing Lipid Peroxidation （LPO） in cerebral cortex and maintaining ceils membrane stability after CPR.