高效纤维素降解菌的筛选及其系统发育分析

SCREENING OF CELLULOSE-DEGRADING FUNGI AND THEIR PHYLOGENETIC ANALYSIS

从刚果红平板上筛选到S41、Z3b、Z28和Z41共4株纤维素酶高产菌株,通过形态观察,生理生化特性分析以及系统进化分析等,鉴定出S41和Z41属于木霉属(Trichoderma sp.),Z3b属于曲霉属(Aspergillus sp.),Z28属于肉座菌属(Hypocrea sp.);4株菌的CMC酶活,FPA酶活和β-葡萄糖苷酶活几乎均高于绿色木霉(Trichderma viride) AS3.2774,葡萄糖对4株菌的β-葡萄糖苷酶均有一定的反馈抑制作用,对Z28的β-葡萄糖苷酶抑制作用较低。

Four fungi named S41, Z3b, Z28 and Z41 were isolated froin soil, capable of producing cellulase with high activity. According to their phenotypic features, physiological and biochemical characteristics, and phylogenetic analysis based on the sequence of 18S rDNA, ZA1 and S41 was identified as Trichoderrna sp., Z3b was identified as Aspergillus sp., Z28 was identified as Hypocrea sp. The activity of CMC, FPA, and β-glucosidase of the isolates is almost higher than that of Trichderma viride AS3. 2774. Still, studies with tolerance of β-glucosidase to glucose found that β-glucosidase produced by Z28 is the most highly glucose-tolerant of the four fungi.