摘要
利用来源于四倍长穗偃麦草(Elytrigiaelongatum4X)的六倍体小偃麦8801(AABBEE)与八倍体小偃麦远中2杂交,通过F1花粉母细胞减数分裂染色体行为分析,发现杂种F1中染色体组主要构型为14Ⅱ+21I(占观察细胞的40%左右),平均构型为:0.89II+12.53I+21.07I,从而确认亲本八倍体小偃麦远中2染色体组中不含有偃麦草的E组染色体。并初步认为远中2染体组型为AABBDDXX。染色体荧光原位杂交(FluorescenceInSituHybridization,FISH)技术检测结果表明:远中2的染色构成是由44条普通小麦染色体、10条中间偃麦草染色体和一对小麦/中间偃麦易位染色体共同组成。并分析了这种组型产生的原因及遗传上的稳定性。
he reciprocal crosses between Yuanzhong 2,an octoploid Trititrigia derived from Elytrigia intermedium and 8801(AABBEE),a line of hexaploid Trititrigia derived from Elytrigia elongatum (4X) were conducted.Based on the analysis of meiosis behavior of pollen mother cell of F1 plant,the main chromsome pairing configuration is 14Ⅱ+2Ⅰ(taking up about 40% of observed cells).The maximum number of bivalent chromosome is only 14.The averagen chromsome pairing configuration is 0.89Ⅲ+12.53Ⅱ+21.07Ⅰ.Thereby,we can confirm that the genome of Yuanzhong 2 exclude E genome derived from Elytrigia intermedium.We can preliminarily concluded the chromosome patten of Yuanzhong 2 is AABBDDXX.The technic of fluorescence in situ hubridization(FISH) was employed to detect the chromosome of Yuanzhong 2.The result indicated that the genome of Yuanzhong 2 is composed of 44 chromosomes from Triticum aestivum,10 chromosomes from Elytrigia intermedium and a pair of translocated chromosomes of triticum aestivum/Elytrigia intermedium.
出处
《哈尔滨师范大学自然科学学报》
CAS
1998年第2期80-85,共6页
Natural Science Journal of Harbin Normal University
基金
黑龙江省科委资助
关键词
八倍体小偃麦
小麦
染色体
荧光原位杂交
Triticum seet Trititrigia octoploid
Triticum seet Trititrigia Hexaploid
Chromosome
FISH