硫丹降解菌的筛选及降解性能研究

Isolation and Identification of Endosulfan Degrading Bacterium C7 and Its Degradation Characteristics

从生产硫丹的农药厂的活性污泥中,分离到一株能以硫丹为唯一碳源生长的细菌C7,经鉴定为苍白杆菌属(O chrobacterumsp.)。构建系统发育树了解C7与其它菌株之间的亲缘关系。用高效液相色谱法对C7的降解性能分析表明,C7在8天内对50 mg/L硫丹的降解率达90.2%以上;菌株的培养条件为pH7,温度30℃,接种量25%时测定降解性能最好。

The strain C7 was isolated from activated sludge, which was identified to be Ochrobacterum sp.. It was cultured with endosulfan as the sole carbon source. The degradative ability of C7 to endosulfan was determined by HPLC, and the degradative rate of endosulfan at 50 The optimum conditions for endosulfan degradation by C7 were 25% mg/L was above 90.2% within 8 days. of total inoculum size, 30℃ and pH 7.0.