大鼠脑出血后行为学、外周血白细胞与脑水肿的相关性研究

Study on the relationship between behavior,peripheral blood leukocyte and cerebral edema after intracerebral hemorrhage in rats

目的研究大鼠脑出血(ICH)后行为学、外周血白细胞和脑水肿的变化规律,并探讨其关系。方法65只SD大鼠随机分为正常对照组、假手术组和ICH组,应用肝素化Ⅶ型胶原酶建立大鼠ICH模型,全自动血球计数仪检测白细胞,Berderson评分评定行为学变化,干/湿重法测定脑含水量。结果(1)大鼠ICH后行为学改变与脑水肿演化规律一致,从12h开始升高,48h达高峰,72h仍维持在较高水平,第7天大致恢复至正常。(2)ICH组外周血白细胞计数12h开始升高,72h达到高峰,48-72h与假手术组和正常对照组比较有显著性统计学意义(P<0.01),随后逐渐下降至正常水平,与脑水肿和行为学变化相一致。结论ICH后行为学改变、外周血白细胞变化能够反映脑水肿的严重程度和病情的轻重。

Objective To study the changes of behavior, peripheral blood leukocyte and cerebral edema after experimental intracerebral hemorrhage （ICH） in rats, and to discuss their relationship. Methods 65 rats were divided into normal control group, sham operated group and ICH group. The ICH model in rats was established by heparinized Ⅶ - type collagenase, automatic blood cell counting instrument to analysis peripheral blood leukocyte, Berderson score to evaluate behavioral change, the dry/wet weight method to check up cerebral water content. Results （ 1 ） The changes of behavioral change and cerebral edema was identical after ICH in rats, 12h beginning to heighten, 48h to achieve peak and 72h still to increase, 7d nearly normal. （2） In ICH group, the numbers of peripheral blood leukocyte begin to heighten, 72h to achieve peak, 48 -72h obviously statistic significance with normal control group and sham operated group （P 〈 0.01 ） , then decreased gradually to normal level. The change was identical with behavioral change and cerebral edema. Conclusion The change of behavioral and peripheral blood leukocyte after ICH in rats can reflect serious degree of cerebral edema and state of illness.