摘要
目的建立一种简便、经济、可靠的血液细胞分析室内质控方法。方法将SysmexSE-9000血细胞分析仪测定系统确定为规范操作检测系统,每日用新鲜血液执行其他血液细胞分析仪与规范操作检测系统的对比试验,将偏差作为评估室内质控的参数。结果对比样本5个参数的测定值涵盖了低、中、高3个水平。12个月WBC、RBC、Hb、HCT、PLT的平均偏差(%)x±s分别为:3.97±3.00、2.13±1.58、1.89±1.55、2.19±1.49、4.64±3.72。常规血细胞分析仪KX-21A与规范操作检测系统间测定新鲜血液的结果符合性良好(r>0.950,P=0.00)。以CLIA′88分析质量要求为允许误差(Ea),WBC、RBC、Hb、HCT、PLT偏差小于1/2Ea的结果百分比分别为:86.6%(310/358)、73.7%(264/358)、84.4%(302/358)、67.9%(243/358)、95.0%(340/358)。结论每日执行新鲜血液的对比试验,即可以实现实时监控发现偶然误差,定期对偏差结果的分析也可以发现系统性偏离或系统误差。新鲜血液对比试验是一种简便、经济而可靠的血细胞分析室内质控方法。
Objective To establish a simple,economical and reliable method for IQC of blood cell count. Methods Sysmex SE-9000 hematology analyzer was defined as a reference analyzer. The fresh blood samples were measured on reference analyzer and other routine analyzers（such as KX-21A hematology analyzer） every day,the comparability and bias were compared. Results The relative bias of WBC,RBC,Hb,HCT and PLT were （x±s）% ：3. 97±3.00,2. 13±1. 58,1.89±1. 55,2. 19±1.49 and 4. 64±3.72, respectively. The results of fresh blood samples that measured by KX-21A hematology analyzer were in correspondence with reference analyzer（Pearson correlation r〉0. 950,P= 0.00）. The acceptable performance of CLIA＇88 was defined as the allowable error（Ea）. The percentage of amount that the bias was lower than 1/2Ea of WBC,RBC, Hb, HCT and PLT were 86. 6% （310/ 358） ,73.7% （264/358） ,84. 4% （302/358）, 67. 9% （243/358） and 95.0%（340/358）, respectively. Conclusion The comparative test with fresh blood is a simple and economical and reliable method for IQC of blood cell count.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第2期180-181,183,共3页
Chongqing medicine
