检测猪肺炎支原体抗体间接ELISA方法与单抗阻断ELISA方法的对比试验

Detection of Mycoplasma hyopneumoniae antibody indirect ELISA method and blocking monoclonal antibody ELISA method of comparative test

猪肺炎支原体(Mycoplasma hyopneumoniae,Mhp)是猪地方性肺炎(Swine Enzootic Pneumonia,SEP)(亦称猪气喘病)的病原体。此病分布广,发病率高,自身致死率不高。由于Mhp能够破坏呼吸道黏膜-纤毛屏障,从而继发其他致病菌的感染,提高致死率。由于早期检测的困难和缺乏有效的药物,在疾病控制方面至今未能达到满意的效果。本实验以Mhp168株为材料,研制出其特异性单克隆抗体,并利用Mhp的全菌蛋白作为包被抗原建立了间接ELISA方法,且证明有一定的实用性。

Mycoplasma hyopneumoniae （Mhp） is the causative agent of Swine Enzootic Pneumoniae （SEP）, a disease found on pig farms worldwide which is characterized by high morbidity and low mortality rates. Since Mhp can compromise the mucociliary clearance mechanism of respiratory, thus predispose the pigs to secondary pulmonary infections and increase the mortality. Now, the early detection of Mhp infection is difficult and there is also no effective drugs to control prevail of this disease. In this experiment, Mhp strain 168 was selected to prepare monoclonal antibodies, which should be potentially used for Mhp infection. And screen a method to detect Mhp by enzyme-linked immunosorbent assay.