视黄素诱导的癌细胞凋亡及其对癌细胞生长抑制的相关性

Study on the relationship between Apoptosis and Growth Inhibition Induced by Retinoids in Cancer Cells

以不同的受体选择性视黄素处理乳腺癌细胞，通过荧光显微术、ＴｄＴ测定和细胞生长测定，分析视黄素诱导的癌细胞凋亡及其对癌细胞生长抑制的相互关系；将受体ＲＡＲα基因转染乳腺癌ＭＢ－２３１细胞，发现受体转染与细胞凋亡诱导有关；ＲＡＲ和ＲＸＲ受体选择性视黄素联合使用，对诱导癌细胞凋亡和抑制癌细胞生长具有加强作用．结果证实，视黄素能够诱导乳腺癌细胞的细胞凋亡，并与抑制癌细胞的恶性生长相一致，这可能是视黄素抗癌作用的机制之一．

Retinoid exerts its effect through two types of retinoid receptors, the retinoic acid receptors(RARs) and retinoid X receptors(RXRs). In this study, we investigated the relationship between apoptosis and growth inhibition induced by RAR and RXR selective retinoids in breast cancer cell lines, using fluorescent microscopy,TdTassay and MTT assay. In addition, RARα gene was stable transfected into RARα negative breast cancer cell line, MB 231 cells, apoptosis was induced. A synergistic growth inhibitory effect and apoptosis were observed on combining RAR and RXR selective retinoids. Our results demonstrate that retinoids can induce apoptosis in breast cancer cell lines, and contribute to growth inhibition of breast cancer cells.These may represent an important anti tumor mechanism of retinoic acid.