摘要
运用差异展示(DD-RT-PCR)方法对野败型(wild abortion)细胞质雄性不育系珍汕97A及其相应的保持系珍汕97B基因产物进行比较分析,发现这两个核基因组完全相同的品系间约有10%的基因表达有差异.选取一个差异表达明显的cDNA成员B132进行North-ern杂交发现,该基因在保持系中正常表达,在不育系中表达受到明显抑制,而在杂种一代中又正常表达,在恢复系中表达水平也很低,这是一种典型的核质互作现象.Southern杂交中,B132在HindⅢ和BamHⅠ酶切长度片段上,各品系间未呈现多态性.
About 10% of the whole genes express differently between rice CMS-WA line Zhenshan 97A and its conservative line Zhenshan 97B detected by DD-RT-PCR analysis. Furthermore, gene B132, gives a typical expression pattern of interaction between nucleus and cytoplasm. Compared to its expression in line B, gene B132 is highly inhibited in line A, while it shows common expression as Line B in F1, and its expression in restorative line is al- so as low as that in line A. B132 doesn't show RFLP within different lines in Southern hybridization patterns.
出处
《复旦学报(自然科学版)》
CAS
CSCD
北大核心
1998年第2期163-167,共5页
Journal of Fudan University:Natural Science
基金
国家科委攀登计划资助课题
关键词
细胞质雄性不育
核质互作
基因表达
野败型水稻
DD-RT-PCR
cytoplasmic male sterile
interaction between nucleus and cytoplasm