核酶的化学合成及其对烟草花叶病毒RNA片段的体外剪切

Chemical Synthesis of Ribozyme and Its Cleavage Activity to TMV-RNA in vitro

设计并用化学方法合成了针对烟草花叶病毒ＲＮＡ的核酶Ｒｚ－１及Ｒｚ－１小型化的核酶Ｒｚ－２以及它们的底物ＲＮＡ片段Ｓｂ．研究了两种核酶对底物Ｓｂ的体外催化活性，并把核酸Ｒｚ－１与生物合成的相同组成的核酶Ｒｚ－１’进行了比较试验．

The ribozyme Rz-1,minimized ribozyme Rz-2 which was modified from Rz-1 and their substrate Sb,a target RNA fragment of tobacco mosaic virus were designed and synthesized by chemical method. The results indicated that both the Rz-1 and Rz-2 showed cleaving activities on their substrates in vitro. The cleaving activity of ribozyme Rz-1 was also compared with that of ribozyme Rz-1' which was synthesized by transcription.