云南西双版纳傣族人群HBV基因型及亚型分布(in English)

Study on genotypes and subgenotypes of hepatitis B virus among Dai Nationality in Xishuangbanna,Yunnan Province

目的研究云南西双版纳傣族HBV感染人群基因型及基因亚型分布找出其优势基因型及基因亚型。方法采用型特异性引物巢式PCR结合聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)对HBV感染者基因型及亚型分型鉴定。结果B基因型58%(25/43),C基因型16%(7/43),B+C基因型21%(9/43),B+E型5%(2/43),无A、D和F型:Ba亚型100%(16/16),Cs亚型50%(2/4),Ce亚型50%(2/4)。结论云南西双版纳傣族人群HBV基因型以B型为优势基因型Ba为优势基因亚型,国内首次发现B+E型,可能为研究其他民族HBV感染提供理论依据。

Objective To investigate the distribution of genotypes and subgenotypes of hepatitis B virus （HBV） among the population of Dai Nationality in Xishuangbanna ,Yunnan Province. Methods The detection of HBV markers were conducted by enzymelinked immunosorbnent assay（ELISA）. HBV genotypes and subgenotypes of the serum samples were tested by PCR-RFLP. Results The genoty pe B was detected in 58% （25/43） of subjects ,genotype C in 16%（7/43）, genotype B＋C in 21%（9/43）, genotype B＋E in5%（2/43）. There were no genotypes of A,D and F. All of B genetypes blonged to subgenotype Ba and subgenotype Bj was not found. In 4 samples with genotype C, 2 （50%） were subgenotype Cs , 2 （50%） were subgenotype ce. Conclusion HBV genotype B is the predominant genotype and subgenotype Ba is the predominant subgenotyoe in Xishuangbanna.This is the first time to discover the B＋E or E genotypes in China .This finding provides a new information for understanding the distribution of HBV genotype in China and a basis to establish Chinese gene bank.