酿酒酵母中一种新的反义snoRNA分子的鉴定(英文)

Identification of A Novel Antisense Small Nucleolar RNA from Yeast

核仁小分子ＲＮＡ（ｓｎｏＲＮＡ）是一类在真核生物核糖体生物合成过程中起重要作用的小分子ＲＮＡ．通过计算机直接分析国际分子生物学数据库及ＲＮＡ杂交分析、ｃＤＮＡ序列测定等方法，在酿酒酵母（Ｓａｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ）中发现和鉴定了１个新的ｓｎｏＲＮＡ－Ｚ６ｓｎｏＲＮＡ．该ｓｎｏＲＮＡ长１０９个核苷酸，由位于酿酒酵母第１３号染色体上的１个独立基因编码．Ｚ６ｓｎｏＲＮＡ含有ｂｏｘＣ（ＵＧＡＵＧＡ）、ｂｏｘＤ（ＣＵＧＡ）等保守的结构元素，属于反义ｓｎｏＲＮＡ家族，即分子中有１段１１个核苷酸的片段与２５ＳｒＲＮＡ中１段保守核心序列互补，该ｓｎｏＲＮＡ片段连同其下游的ｂｏｘＤ共同指导与其互补的ｒＲＮＡ序列第２１９５位胞苷酸的２’－氧－核糖的甲基化．

A novel small nucleolar RNA, Z6, was identified and characterized from yeast Saccharomyces cerevisiae. Z6 snoRNA is 109 nt. in length, encoded by an independent transcribed gene which probably belongs to a snoRNA gene cluster on the chromosome ⅩⅢ of yeast. It contains boxC, boxD motifs and a 11 nt. long segment complimentary to a conserved sequence of 25S rRNA. The antisense element, together with the downstream boxD’, guides a 2’ O ribose methylation of cytidine acid at position 2195 in yeast 25S rRNA.