利用RAPD标记鉴定芥菜(Brasica juncea Cos.)品种(英文)

Identification of Mustard( Brassica juncea Coss.) Cultivars with RAPD Markers

从６０种１０ｂｐ随机引物中筛选出７种引物，对８个芥菜变种的１６个品种（每个变种有２个品种）进行了随机扩增多态性ＤＮＡ（ＲＡＰＤ）鉴定，能快速、灵敏、较为简便获得了１６个芥菜品种的基因组ＤＮＡ指纹图谱．结果表明，不同引物在１６个品种上都有其特征ＤＮＡ带，一般只有１条，少数有２条．在同１个变种的２个品种之间存在着丰富的ＲＡＰＤ标记，通过比较２个品种各自独具的ＲＡＰＤ标记，就可明确地将２个品种区分．在所用的７种引物中，没有１种能同时将８对芥菜品种全部区分开，但用２种或２种以上的引物就完全可以将８对芥菜品种全部区分开．

Random amplified polymorphic DNA(RAPD) is a new molecular marker technology developed by Williams and Welsh in 1990.Sixteen mustard( Brassica juncea Coss.)cultivars of eight varieties were identified with RAPD markers. Seven 10 mer arbitrary primers were selected out of sixty one, and genomic DNA fingerprints of sixteen mustard cultivars were obtained. One or two characteristic DNA bands were shared by each primer among sixteen cultivars. RAPD markers between two mustard cultivars of one variety were rich and two cultivars could be distinguished through comparing the different DNA bands between them. No single primer out of seven could distinguish all the eight pair cultivars on one analysis while combination of two or more primers could do it. The results showed that identification of mustard cultivars with RAPD markers is a sensitive and rapid approach technology which can be applied widely in seed scientific researches and seed production.