摘要
目的:探讨C3d与C3d-IC的测定方法及其与临床疾病的关系。方法:用抗人C3和抗人IgGγ-球蛋白组份作为固相反应物,建立了检测C3d及C3d-IC的ELISA法,并对临床各类疾病患者血清C3d与C3d-IC的水平进行了测定。结果:发现慢性肾炎、全身性红斑狼疮(SLE)、慢性乙型肝炎及肺炎患者血清中C3d总体水平均较对照组显著增高,分别有58.5%~72.2%的患者C3d含量高于正常上限(P<0.01),且该类患者亦有较高的C3d-IC阳性检出率(肺炎患者除外)。本研究表明,C3d-IC阳性者,其血清C3d含量均高于正常水平,二者具有显著的相关性。结论:C3d-IC与补体的起始激活有直接关系,因而C3d-IC与C3d可以看作是疾病或组织损伤及补体活化反应的重要参考指标。
Objectives:To investigate the associations between C3d and C3dIC as well as clinical diseases. Methods:Using antihuman C3 and antihuman IgGγ globulin as solid phase reagents,an enzyme linked immunosorbent assay(ELISA) for C3d and C3dIC was developed,and was used to evaluate sera from patients with variety of clinical diseases,for the levels of C3d and C3dIC. Results:The total levels of sera of C3d which from patients with chronic nephritis,SLE,chronic HBV hepatitis and pneumonia was significantly elevated than control.It was 58.5%~72.2% of patients that sera C3d concentrations exceeded the upper limit of normal respectively(P<0.01),and the positive rate of C3dIC was also higher than control(except pneumonia).In this study,all of C3dIC positive cases had a higher levels of C3d,and both of them had significant associations. Conclusions:C3dIC was directly related to the activation of complement cascade.Therefore,C3dIC and C3d could serve as important index causing diseases and tissue damage as well as activation of complement reflection.
出处
《南京大学学报(自然科学版)》
CAS
CSCD
1998年第2期164-167,共4页
Journal of Nanjing University(Natural Science)