非小细胞肺癌外周血CD4^+CD25^+FoxP3^+调节T细胞分析

Study on the CD4^+CD25^+FoxP3^+ Regulatory Cells in Peripheral Blood of NSCLC Patients

目的分析非小细胞肺癌(NSCLC)患者外周血CD4+CD25+FoxP3+调节T细胞(Treg)占CD4+T细胞的比例变化,探讨其临床意义。方法采用流式细胞仪检测32例非小细胞肺癌患者和15例正常健康者(对照组)外周血中CD4+CD25+FoxP3+Treg占CD4+T细胞的比例。结果健康对照组外周血CD4+CD25+FoxP3+Treg占CD4+T细胞比例为(0.54±0.51)%;NSCLC患者外周血CD4+CD25+FoxP3+Treg占CD4+T细胞比例为(1.40±0.98)%。两组比较差异有统计学意义(P<0.01)。结论NSCLC患者外周血CD4+CD25+FoxP3+Treg占CD4+T细胞的比例明显升高,提示与肺癌免疫逃逸有关。

Objective To study the frequency of the CD4^＋CD25^＋FoxP3^＋ regulatory T cells（Treg） in the patients with nonsmall cell lung cancer（NSCLC） and its clinical significance. Methods The percentage of the CD4^＋ CD25^＋ FoxP3^＋ Treg cells in the peripheral blood from 32 patients with NSCLC and 15 healthy controls detected by flow cytometry. Results CD4^＋ CD25^＋ FoxP3^＋ Treg cells from patients with NSCLC were significantly increased, compared with those from the control, they were （0.54±0.51）% and（1.40±0.98）% respectively（P〈0.01）.Conclusion There is an inereasing of the proportion of CD4^＋ CD25^＋ FoxP3^＋ Treg cells in peripheral blood of the patients with NSCLC, which probably may be related to immuno-tolerance with NSCLC, suggesting depletion of the CD4^＋ CD25^＋ FoxP3^＋ Treg cells may be an effective strategy for NSCLC patients.