摘要
目的通过测定急性缺血性脑卒中患者血清S-100β蛋白的变化,探讨其与梗死灶大小、神经学状态和神经学预后间的关系。方法采用双抗体夹心酶联免疫吸附试验(ELISA)检测急性缺血性脑卒中患者发病后48 h内、第5天、第7天和第14天血清S-100β水平。所有患者于相应时间用美国国立卫生研究院卒中量表(NIHSS)进行神经学状态评估,并于出院时评估神经学功能(B I)。结果患者组各时间点S-100β水平与对照组差异均无统计学意义。S-100β于所有时间点,较大、中等和较小梗死灶组及对照组差异有统计学意义(P<0.05),且与相应NIHSS和出院时B I均有非常明显的相关性(P=0.000)。较大梗死灶组血清S-100β水平较高。结论S-100β水平在缺血性脑卒中患者中明显升高,有望成为缺血性脑卒中早期评估、揭示疾病严重程度和评估预后的指标。
Objective To determine the changes of serum S-100βprotein level in patients with acute cerebral ischemia and investigate the relationship with the level and size of brain lesions, neurological status and functional outcome. Methods The serum levels of S-100β protein in patients with acute cerebral ischemia at 48 h, 5,7 and 14 d after stroke were determined by enzyme-linked immunosorbent assay（ ELISA）. Neurological status of all patients was evaluated by National Institutes of Health Stroke Scale （NIHSS） at corresponding time, and functional outcome was evaluated by Barthel Index （BI） at discharge from hospital. Results The levels of S-100β protein in patients with cerebral ischemia at any time had no significant difference with control group. For all time points,the level of S-100β protein in had significant difference among patients with large, middle, small size of cerebral infarction and control group（ P 〈 0.05 ） , and significant correlation between S-100β protein level and corresponding NIHSS and BI at discharge （ P = 0. 000）. The level of S-10013 protein in patients with large size of infarction was higher. Conclusions The levels of S-100β protein in patients with cerebral ischemia increase markedly. S-100β protein may be an useful marker for diagnosis and prognosis in patients with cerebral ischemia.
出处
《检验医学》
CAS
北大核心
2009年第1期33-36,共4页
Laboratory Medicine
基金
北京市城区科技型中小企业创业资金资助项目(04041)
北京市西城区优秀人才专项经费联合资助项目(041227)
