摘要
目的首次建立火麻仁中大麻酰胺A的含量测定方法。方法采用Waters C18（250mm×4．6mm，5μm）色谱柱；乙腈-0．1％磷酸溶液（22：78）为流动相；流速：1．0mL·min^-1；检测波长：257nm。结果大麻酰胺A的线性范围0．1～0．50μg（r=0．9996）；加样回收率为101．16％，RSD=1．63%（n=6）。结论该法操作简便、准确、重复性好，可用于火麻仁的质量控制。
Objective To establish an HPLC method to determine the content of Cannabisin A in fruits of Cannabis Sativa. Methods The HPLC system consisted of Waters column Cls （250 mm× 4. 6 mm, 5 μm） ; acetonitrile -0. 1% phosphoric acid （22 ： 78） as the mobile phase; flow rate was 1.0 mL · min 1. It was detected at 257 nm. Results The linear range of Cannabisin A was 0. 1-0.50 μg, r=0. 999 6; the mean recovery was 101.16%, RSD= 1.63% （n=6） . Conclusion This method is simple and accurate with good reproducibility. It is suitable for the quality control of fruits of Cannabis Sativa.
出处
《中南药学》
CAS
2009年第1期26-28,共3页
Central South Pharmacy
