湿邪对不同梯度的寒邪犯肺大鼠细胞因子变化影响

Effect of Pathogenic Dampness on Changes of Cytokines of Rats with Different Gradients of Evil-Cold Invading Fei

目的研究湿邪对不同梯度的寒邪犯肺所导致的大鼠肺组织匀浆中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素6(interleukin-6,IL-6)、白细胞介素4(interleukin-4,IL-4)、γ干扰素(interferon-γ,IFN-γ)等细胞因子水平变化的影响。方法将104只SPF级Wistar大鼠按随机数字表法分为13组,每组8只:常温对照组、寒邪6组和寒湿(寒邪夹湿邪)6组,寒邪分为恒定低温与温差两种。其中恒定低温再分为10℃、0℃、-10℃3个不同梯度组,温差组分为20～10℃、20～0℃、20～-10℃3个不同梯度组。第1天将大鼠置于相应环境的调温箱中饲养2h后取出,置于常温饲养,观察大鼠一般状况,4天后取材。检测肺组织匀浆中TNF-α、IL-6、IFN-γ、IL-4的含量。结果与相同温度的寒邪组比较,各寒湿组大鼠肺组织匀浆中TNF-α和IL-6水平均有不同程度的显著上升(P<0.01),IFN-γ水平显著下降(P<0.01),IL-4水平显著上升(P<0.05或P<0.01),IFN-γ/IL-4比值显著下降(P<0.01)。结论湿邪能够明显加重寒邪对肺脏的损害,从而引起炎症相关细胞因子不同程度的变化,且湿邪能明显加重寒邪犯肺所导致的机体Th1/Th2免疫应答不同程度的失衡。

Objective To research the influence of pathogenic dampness on changes of cytokines such as tumor necrosis factor-α （TNF-α）, interleukin-6 （IL-6）, interluekin-4 （IL-4）, interferon-y （IFN-γ）, etc. in the lung of rats with different gradients of evil-cold invading Fei. Methods One hundred and four Wistar rats of SPF grade were randomized depending on the digital table into 13 groups, 8 in each group： the normal temperature group, the 6 evil-cold invasive groups （ECG1-6） and the 6 cold-dampness invasive groups （CDG1-6）. Rats in the ECG and CDG groups were exposed to cold or cold-dampness to different gradients, for subgroups 1 to 6, - 10℃, 0℃, 10℃, 20 -10℃, 20 -0℃ and 20 - -10℃ was applied respectively for2 h, then fed at room temperature to observe their general condition. Rats were sacrificed 4 days later and their lung tissue was taken out for determination of the contents of TNF-α, IL-6, IFN-γ and IL-4 in lung tissue homogenate. Results Compared with the corresponding ECG groups, the contents of TNF-α, IL-6 and IL-4 were higher （P〈0.05 or P〈0.01 ）, content of IFN-γ and IFN-γ/IL-4 ratio were significantly lower in the CDG groups （P 〈 0.01 ）. Conclusion - Pathogenic dampness could aggravate the injury of Fei caused by the pathogenic cold to induce the change of inflammation related cytokines to a certain extent, and the unbalance of Th1/Th2 immune response in organism could be worsened as well.