麻风病流行区水、土壤中麻风杆菌的检测初步报告

Detection of M. leprae DNA from water and soil in a leprosy endemic area - a primary report

目的：检测云南省丘北县水、土壤中是否存在麻风杆菌，探究水、土壤是否充当麻风杆菌病原库。方法：从麻风病不同流行程度的村庄采集水、土壤，从中分离出微生物、提取DNA；Nested—PCR扩增后直接测序，用基本局域线性搜索工具（BLAST）与NCBIGenBank中的标准序列进行比较，以确认PCR产物是否为麻风杆菌。结果：从36个采样点采集的59份水样中，有29份PCR阳性，其中18份标本测序后能确认为麻风杆菌。从7个采样点采集的11份土样中有7份PCR阳性，其中3份标本测序后能确认为麻风杆菌。结论：麻风病流行村庄水、土壤中存在的麻风杆菌可能是麻风病的传染源。

Objective： % detect whether M. leprae is present in the environment, and explore whether soil and water act as a reservoir for M. leprae. Methods： Water and soil samples were collected from different leprosy endemic villages, and DNA was extracted and amplified by Nested- PCR with M. lepraespecific primers. The PCR products were sequenced and compared with those obtained in NCBI GenBank by basic local alignment search tool （BLAST）. Results： The targeted DNA fraganents were amplified by PCR from 28 out of 59 water samples collected from 36 different locations. Among the 28 samples, M. leprae was identified by BLAST in 18 samples. Seven of 11 soil samples collected from 7 different locations and M. leprae was identified by BLAST in 3 of 4 samples. Conclusion： M. leprae presented in water and soil might be the cause of transmission of leprosy.