变应性接触性皮炎患者外周血调节性T细胞的检测

Detection of regulatory T cells in peripheral blood of patients with allergic contact dermatitis

目的：检测变应性接触性皮炎（ACD）患者外周血CD4^＋CD25^＋CD127^-调节性T细胞（Treg）的表达。方法：选取斑贴试验确证镍为主要过敏原的ACD患者，采用流式细胞仪检测36例患者和30例正常人外周血的Treg。结果：ACD患者外周血Treg占总T细胞的百分比较对照组明显下降（P〈0．05）。结论：Treg的百分比下降，对变应性接触性皮炎的发病有重要影响。

Objective： To investigate the expression of CD4^＋ CD25^＋ CD127^- regulatory T cells （Tregs） in the peripheral blood of the patients with allergic contact dermatitis （ACD）. Methods： Tregs in peripheral blood in 36 patients with allergy to nickel confirmed by patch test and 30 normal controls were tested by three - color flow cytometry. Results： The average percentage of Tregs was decreased significantly in patients with ACD compared with that of norreal controls （ P 〈0.05）. Conclusion： Tregs in peripheral blood of patients may play an impoitant role in the pathogenesis of ACD.